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KMID : 0350519950480020503
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Journal of Catholic Medical College
1995 Volume.48 No. 2 p.503 ~ p.513
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Effects of Epidermal Growth Factor on the Mature Rat Prostate after Sialoadenectomy or Orchiectomy
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Abstract
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Epidermal growth factor (EGF) is a polypeptide hormone which is produced in large amounts in the submandibular glands under the influence of androgen. Sialoadenectomy causes plasma EGF to reach undetectable levels by three weeks. A number of
studies
have documented that EGF plays an important role in maintaining the integrity of the male accessory sex organs. We have undertaken this investigation to determine the effects of EGF on the rat dorsolateral lobe of prostate and influences of
andregen in
prostatic EGF level after sialoadenectomy or orchiectomy. 45 mature Sprague-Dawley rats were divided into three groups with 15 rats in each ; 1) sham operated control group 2) sialoadenectomy (Sx) group 3) orchiectomy (Ox) group. All animals were
sacrificed at 4 weeks and prostate weights, serum testosterone levels, serum LH and FSH levels, EGF levels in dorsolateral lobe of prostate were measured, and changes of histologic and immunohistochemical findings were observed by light
microscopy.
@ES The results were as follows :
@EN 1. The prostate weights of Sx group were significantly lower than those of control group (p<0.05). In Ox group, the prostate weights were significantly lower than those of control and Sx group (P<0.05).
2. In Sx group, serum testosterone levels were significantly increased and serum LH levels were decreased than those of control group (P<).05). There were no statistical differences in serum FSH levels between control and Sx group. In Ox group,
serum
LH and FSH levels were greater than those of control group(P<0.05).
3. EGF levels in prostate of Sx and Ox group were significantly decreased than these of cnotrol group as 6.7±1.2 ng/mg tissue and 12.4±2.0 ng/mg tissue (P<0.05).
4. In control group, much of the eosinophilic secretion is accumulated within the glandular lumina and only luminal fluid is intensely positive in immunostainng for EGF. There is moderated glandular atrophy (P<0.05) with decreased luminal
secretion and
the immunostaining is considerably reduced in Sx group. In Ox group, more severe atrophy was noted, but immunostaining is more intense than that of Sx group.
These results suggest that EGF plays an important role in maintaining the integrity of the prostate in the mature rat and the amount of EGF in prostate is regulated by androgen.
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KEYWORD
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